Chapter title |
Vaccine Design
|
---|---|
Chapter number | 31 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Deng, Lei, Linero, Florencia, Saelens, Xavier, Lei Deng, Florencia Linero, Xavier Saelens |
Editors |
Sunil Thomas |
Abstract |
Viruslike particles often combine high physical stability with robust immunogenicity. Furthermore, when such particles are based on bacteriophages, they can be produced in high amounts at minimal cost and typically will require only standard biologically contained facilities. We provide protocols for the characterization and purification of recombinant viruslike particles derived from filamentous bacteriophages. As an example, we focus on filamentous Escherichia coli fd phage displaying a conserved influenza A virus epitope that is fused genetically to the N-terminus of the major coat protein of this phage. A step-by-step procedure to obtain a high-titer, pure recombinant phage preparation is provided. We also describe a quality control experiment based on a biological readout of the purified fd phage preparation. These protocols together with the highlighted critical steps may facilitate generic implementation of the provided procedures for the display of other epitopes by recombinant fd phages. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 3 | 23% |
Student > Ph. D. Student | 2 | 15% |
Researcher | 2 | 15% |
Professor | 1 | 8% |
Lecturer > Senior Lecturer | 1 | 8% |
Other | 2 | 15% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 23% |
Agricultural and Biological Sciences | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Immunology and Microbiology | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |