Chapter title |
Vaccine Design
|
---|---|
Chapter number | 39 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_39 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Boes, Alexander, Reimann, Andreas, Twyman, Richard M, Fischer, Rainer, Schillberg, Stefan, Spiegel, Holger, Alexander Boes, Andreas Reimann, Richard M. Twyman, Rainer Fischer, Stefan Schillberg, Holger Spiegel |
Editors |
Sunil Thomas |
Abstract |
There are currently no vaccines that provide sterile immunity against malaria. Various proteins from different stages of the Plasmodium falciparum life cycle have been evaluated as vaccine candidates, but none of them have fulfilled expectations. Therefore, combinations of key antigens from different stages of the parasites life cycle may be essential for the development of efficacious malaria vaccines. Following the identification of promising antigens using bioinformatics, proteomics, and/or immunological approaches, it is necessary to express, purify, and characterize these proteins and explore the potential of fusion constructs combining different antigens or antigen domains before committing to expensive and time-consuming clinical development. Here, using malaria vaccine candidates as an example, we describe how Agrobacterium tumefaciens-based transient expression in plants can be combined with a modular and flexible cloning strategy as a robust and versatile tool for the rapid production of candidate antigens during research and development. |
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Mendeley readers
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Student > Master | 7 | 39% |
Student > Bachelor | 4 | 22% |
Researcher | 3 | 17% |
Student > Ph. D. Student | 2 | 11% |
Professor > Associate Professor | 1 | 6% |
Other | 0 | 0% |
Unknown | 1 | 6% |
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Biochemistry, Genetics and Molecular Biology | 3 | 17% |
Immunology and Microbiology | 3 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
Medicine and Dentistry | 2 | 11% |
Other | 3 | 17% |
Unknown | 2 | 11% |