Chapter title |
Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
|
---|---|
Chapter number | 8 |
Book title |
Recombinant Protein Expression in Mammalian Cells
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8730-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
Authors |
David I. Fisher, Fisher, David I. |
Abstract |
Large culture volumes are often required when expression constructs are particularly low-yielding or when end-uses require significant amounts of material. In these cases, a single homogenous culture is usually more convenient, in terms of both consistency of expression and labour/resource requirements, than multiple parallel cultures. Using a WAVE Bioreactor culture volumes as high as 500 L may be achieved in a single vessel. Here we describe the transfection of 293-6E cells in a disposable 50 L Cellbag on a WAVE Bioreactor platform to produce recombinant protein. The methods described herein may be adapted, with suitable optimizations, for other suspension-adapted mammalian cell lines. |
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