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Recombinant Protein Expression in Mammalian Cells

Overview of attention for book
Cover of 'Recombinant Protein Expression in Mammalian Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Transient Gene Expression in Suspension HEK293-EBNA1 Cells
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    Chapter 2 Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells
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    Chapter 3 PEI-Mediated Transient Gene Expression in CHO Cells
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    Chapter 4 Stable Expression by Lentiviral Transduction of Cells
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    Chapter 5 Inducible Protein Production in 293 Cells Using the piggyBac Transposon System
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    Chapter 6 Recombinant CHO Cell Pool Generation Using piggyBac Transposon System
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    Chapter 7 Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression
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    Chapter 8 Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
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    Chapter 9 CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
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    Chapter 10 Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
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    Chapter 11 Continuous and Integrated Expression and Purification of Recombinant Antibodies
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    Chapter 12 High Throughput Transfection of HEK293 Cells for Transient Protein Production
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    Chapter 13 Microfluidic Transfection for High-Throughput Mammalian Protein Expression
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    Chapter 14 Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production
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    Chapter 15 Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells
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    Chapter 16 Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
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    Chapter 17 Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)
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    Chapter 18 Considerations in the Use of Codon Optimization for Recombinant Protein Expression
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    Chapter 19 Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells
Attention for Chapter 5: Inducible Protein Production in 293 Cells Using the piggyBac Transposon System
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Chapter title
Inducible Protein Production in 293 Cells Using the piggyBac Transposon System
Chapter number 5
Book title
Recombinant Protein Expression in Mammalian Cells
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8730-6_5
Pubmed ID
Book ISBNs
978-1-4939-8729-0, 978-1-4939-8730-6
Authors

Iacovos P. Michael, Andras Nagy, Michael, Iacovos P., Nagy, Andras

Abstract

Recombinant proteins are widely used to study various pathophysiological processes. Nevertheless, the establishment of the desired protein-producing stable mammalian cell lines using traditional methods is hampered by multiple laborious steps. In this chapter, we describe a simple and robust system that allows for the derivation of stable transgenic cell lines in 293 cells, yielding high protein expression levels, in a short time period. This methodology is based on the piggyBac transposon system and, notably, it allows for inducible production of the protein of interest. Moreover, it can easily be used in conventional laboratory cell culture settings and does not require any specialized devices. Herein, we outline all the steps of this procedure in detail and point out specific considerations.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 29%
Researcher 2 29%
Student > Master 1 14%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Medicine and Dentistry 1 14%
Unknown 3 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2019.
All research outputs
#17,990,409
of 23,103,903 outputs
Outputs from Methods in molecular biology
#7,322
of 13,222 outputs
Outputs of similar age
#243,878
of 340,695 outputs
Outputs of similar age from Methods in molecular biology
#129
of 235 outputs
Altmetric has tracked 23,103,903 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,222 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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We're also able to compare this research output to 235 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.