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Mendeley readers
| Chapter title |
CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
|
|---|---|
| Chapter number | 9 |
| Book title |
Recombinant Protein Expression in Mammalian Cells
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8730-6_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
| Authors |
David L. Hacker, Laurence Durrer, Soraya Quinche, Hacker, David L., Durrer, Laurence, Quinche, Soraya |
| Abstract |
Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 33% |
| Student > Ph. D. Student | 1 | 8% |
| Unspecified | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 42% |
| Unspecified | 1 | 8% |
| Computer Science | 1 | 8% |
| Chemistry | 1 | 8% |
| Neuroscience | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |