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Recombinant Protein Expression in Mammalian Cells

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Cover of 'Recombinant Protein Expression in Mammalian Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Transient Gene Expression in Suspension HEK293-EBNA1 Cells
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    Chapter 2 Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells
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    Chapter 3 PEI-Mediated Transient Gene Expression in CHO Cells
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    Chapter 4 Stable Expression by Lentiviral Transduction of Cells
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    Chapter 5 Inducible Protein Production in 293 Cells Using the piggyBac Transposon System
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    Chapter 6 Recombinant CHO Cell Pool Generation Using piggyBac Transposon System
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    Chapter 7 Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression
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    Chapter 8 Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
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    Chapter 9 CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
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    Chapter 10 Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
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    Chapter 11 Continuous and Integrated Expression and Purification of Recombinant Antibodies
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    Chapter 12 High Throughput Transfection of HEK293 Cells for Transient Protein Production
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    Chapter 13 Microfluidic Transfection for High-Throughput Mammalian Protein Expression
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    Chapter 14 Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production
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    Chapter 15 Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells
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    Chapter 16 Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
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    Chapter 17 Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)
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    Chapter 18 Considerations in the Use of Codon Optimization for Recombinant Protein Expression
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    Chapter 19 Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells
Attention for Chapter 1: Transient Gene Expression in Suspension HEK293-EBNA1 Cells
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Chapter title
Transient Gene Expression in Suspension HEK293-EBNA1 Cells
Chapter number 1
Book title
Recombinant Protein Expression in Mammalian Cells
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8730-6_1
Pubmed ID
Book ISBNs
978-1-4939-8729-0, 978-1-4939-8730-6
Authors

Denis L’Abbé, Louis Bisson, Christian Gervais, Eric Grazzini, Yves Durocher, L’Abbé, Denis, Bisson, Louis, Gervais, Christian, Grazzini, Eric, Durocher, Yves

Abstract

Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 23%
Student > Doctoral Student 5 13%
Student > Ph. D. Student 3 8%
Student > Bachelor 2 5%
Lecturer 1 3%
Other 5 13%
Unknown 14 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 33%
Chemical Engineering 3 8%
Agricultural and Biological Sciences 3 8%
Engineering 2 5%
Unspecified 1 3%
Other 2 5%
Unknown 15 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2019.
All research outputs
#20,533,782
of 23,103,903 outputs
Outputs from Methods in molecular biology
#9,985
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Outputs of similar age
#296,261
of 340,695 outputs
Outputs of similar age from Methods in molecular biology
#176
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