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Mendeley readers
| Chapter title |
Stable Expression by Lentiviral Transduction of Cells
|
|---|---|
| Chapter number | 4 |
| Book title |
Recombinant Protein Expression in Mammalian Cells
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8730-6_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
| Authors |
Natascha Gödecke, Hansjörg Hauser, Dagmar Wirth, Gödecke, Natascha, Hauser, Hansjörg, Wirth, Dagmar |
| Abstract |
Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome the transgene is stably maintained and long term producing cells are established. Here, we describe the current state of the art and give details for lab scale production of lentiviral vectors as well as for infection and titration of the viral vectors. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 24% |
| Student > Ph. D. Student | 4 | 16% |
| Student > Master | 3 | 12% |
| Student > Doctoral Student | 1 | 4% |
| Other | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 36% |
| Agricultural and Biological Sciences | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Immunology and Microbiology | 2 | 8% |
| Business, Management and Accounting | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 9 | 36% |