Chapter title |
Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
|
---|---|
Chapter number | 16 |
Book title |
Recombinant Protein Expression in Mammalian Cells
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8730-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
Authors |
Qiong Wang, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu, Michael J. Betenbaugh, Wang, Qiong, Chung, Cheng-Yu, Rosenberg, Julian N., Yu, Geng, Betenbaugh, Michael J. |
Abstract |
Genetic engineering plays an essential role in the development of cell lines for biopharmaceutical manufacturing. Advanced gene editing tools can improve both the productivity of recombinant cell lines as well as the quality of therapeutic antibodies. Antibody glycosylation is a critical quality attribute for therapeutic biologics because the glycan patterns on the antibody fragment crystallizable (Fc) region can alter its clinical efficacy and safety as a therapeutic drug. As an example, recombinant antibodies derived from Chinese hamster ovary (CHO) cells are generally highly fucosylated; the absence of fucose significantly enhances antibody dependent cell-mediated cytotoxicity (ADCC) against cancer cells. This chapter describes a protocol applying clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) to disrupt the α-1,6-fucosyltranferase (FUT8) gene and subsequently inhibit α-1,6-fucosylation on antibodies expressed in CHO cells. |
X Demographics
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Unknown | 3 | 100% |
Demographic breakdown
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Ph. D. Student | 6 | 24% |
Student > Bachelor | 3 | 12% |
Researcher | 2 | 8% |
Other | 2 | 8% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 2 | 8% |
Unknown | 9 | 36% |
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Chemical Engineering | 1 | 4% |
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Agricultural and Biological Sciences | 1 | 4% |
Other | 3 | 12% |
Unknown | 8 | 32% |