Chapter title |
Microfluidic Transfection for High-Throughput Mammalian Protein Expression
|
---|---|
Chapter number | 13 |
Book title |
Recombinant Protein Expression in Mammalian Cells
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8730-6_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
Authors |
Kristina Woodruff, Sebastian J. Maerkl, Woodruff, Kristina, Maerkl, Sebastian J. |
Abstract |
Mammalian synthetic biology and cell biology would greatly benefit from improved methods for highly parallel transfection, culturing, and interrogation of mammalian cells. Transfection is routinely performed on high-throughput microarrays, but this setup requires manual cell culturing and precludes precise control over the cell environment. As an alternative, microfluidic transfection devices streamline cell loading and culturing. Up to 280 transfections can be implemented on the chip at high efficiency. The culturing environment is tightly regulated and chambers physically separate the transfection reactions, preventing cross-contamination. Unlike typical biological assays that rely on end-point measurements, the microfluidic chip can be integrated with high-content imaging, enabling the evaluation of cellular behavior and protein expression dynamics over time. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 25% |
Unspecified | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Researcher | 1 | 13% |
Other | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Unspecified | 1 | 13% |
Agricultural and Biological Sciences | 1 | 13% |
Materials Science | 1 | 13% |
Unknown | 3 | 38% |