| Chapter title |
Considerations in the Use of Codon Optimization for Recombinant Protein Expression
|
|---|---|
| Chapter number | 18 |
| Book title |
Recombinant Protein Expression in Mammalian Cells
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8730-6_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
| Authors |
Vincent P. Mauro, Stephen A. Chappell, Mauro, Vincent P., Chappell, Stephen A. |
| Abstract |
Codon optimization is a gene engineering approach that is commonly used for enhancing recombinant protein expression. This approach is possible because (1) degeneracy of the genetic code enables most amino acids to be encoded by multiple codons and (2) different mRNAs encoding the same protein can vary dramatically in the amount of protein expressed. However, because codon optimization potentially disrupts overlapping information encoded in mRNA coding regions, protein structure and function may be altered. This chapter discusses the use of codon optimization for various applications in mammalian cells as well as potential consequences, so that informed decisions can be made on the appropriateness of using this approach in each case. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 25% |
| Researcher | 5 | 18% |
| Student > Ph. D. Student | 2 | 7% |
| Unspecified | 2 | 7% |
| Student > Bachelor | 2 | 7% |
| Other | 1 | 4% |
| Unknown | 9 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 36% |
| Unspecified | 2 | 7% |
| Agricultural and Biological Sciences | 2 | 7% |
| Medicine and Dentistry | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 12 | 43% |