| Chapter title |
Continuous and Integrated Expression and Purification of Recombinant Antibodies
|
|---|---|
| Chapter number | 11 |
| Book title |
Recombinant Protein Expression in Mammalian Cells
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8730-6_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8729-0, 978-1-4939-8730-6
|
| Authors |
Sebastian Vogg, Moritz Kai Florian Wolf, Massimo Morbidelli, Vogg, Sebastian, Wolf, Moritz Kai Florian, Morbidelli, Massimo |
| Abstract |
This chapter introduces the necessary concepts to design continuous expression and purification processes for monoclonal antibodies. The operation of a perfusion bioreactor is discussed containing the preparation procedures, the seeding train and the preparation and control of a long-term production run. The downstream processes exploit the benefits of countercurrent chromatography. Their design from batch experiments is presented. The CaptureSMB process is introduced for continuous capturing while for polishing applications the design of the two-column MCSGP process is described. The chapter also puts these processes together in the context of their integration to an end-to-end production process. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 38% |
| Researcher | 4 | 17% |
| Student > Doctoral Student | 3 | 13% |
| Student > Master | 3 | 13% |
| Student > Bachelor | 2 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 7 | 29% |
| Biochemistry, Genetics and Molecular Biology | 5 | 21% |
| Engineering | 5 | 21% |
| Social Sciences | 2 | 8% |
| Psychology | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 4 | 17% |