Chapter title |
An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Cells
|
---|---|
Chapter number | 10 |
Book title |
Cytoskeleton Methods and Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3124-8_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3123-1, 978-1-4939-3124-8
|
Authors |
Luís Carlos Santos, Emilia Laura Munteanu, Nicolas Biais |
Abstract |
The aim of this chapter is to present an innovative technique to visualize changes of the f-actin cytoskeleton in response to locally applied force. We developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated with the Neisseria gonorrhoeae Type IV pili in the same order of magnitude than the forces generated by live bacteria. We saw quick and robust f-actin accumulation at the sites where pulling forces were applied. Using the magnetic tweezers we were able to mimic the local response of the f-actin cytoskeleton to bacteria-generated forces. In this chapter we describe our magnetic tweezers system and show how to control it in order to study cellular responses to force. |
X Demographics
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United States | 2 | 100% |
Demographic breakdown
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 25% |
Student > Postgraduate | 2 | 25% |
Professor > Associate Professor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Physics and Astronomy | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Engineering | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |