| Chapter title |
Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.
|
|---|---|
| Chapter number | 3 |
| Book title |
Cytoskeleton Methods and Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3124-8_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3123-1, 978-1-4939-3124-8
|
| Authors |
Higuchi-Sanabria, Ryo, Swayne, Theresa C, Boldogh, Istvan R, Pon, Liza A, Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon |
| Abstract |
The budding yeast Saccharomyces cerevisiae is widely used as a model system to study the organization and function of the cytoskeleton. In the past, its small size, rounded shape, and rigid cell wall created obstacles to explore the cell biology of this model eukaryote. It is now possible to acquire and analyze high-resolution and super-resolution multidimensional images of the yeast cell. As a result, imaging of yeast has emerged as an important tool in eukaryotic cell biology. This chapter describes labeling methods and optical approaches for visualizing the cytoskeleton and interactions of the actin cytoskeleton with mitochondria in fixed yeast cells using wide-field and super-resolution fluorescence microscopy. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 36% |
| Student > Ph. D. Student | 2 | 18% |
| Student > Doctoral Student | 1 | 9% |
| Student > Master | 1 | 9% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 55% |
| Agricultural and Biological Sciences | 1 | 9% |
| Chemistry | 1 | 9% |
| Unknown | 3 | 27% |