You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Using a Handheld Gene Gun for Genetic Transformation of Tetrahymena thermophila
|
---|---|
Chapter number | 22 |
Book title |
Cytoskeleton Methods and Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3124-8_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3123-1, 978-1-4939-3124-8
|
Authors |
Michael Gotesman, Selwyn A. Williams |
Abstract |
This chapter describes protocols for using a handheld gene gun to deliver transformation vectors for overexpression of genes or gene replacement in the macronucleus of Tetrahymena thermophila. The protocols provide helpful information for preparing Tetrahymena for biolistic bombardment, preparation of vector-coated microcarriers, and basic gene gun operating procedures. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 50% |
Other | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Business, Management and Accounting | 1 | 50% |