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Chapter title |
Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament Severing and Bundling
|
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Chapter number | 9 |
Book title |
Cytoskeleton Methods and Protocols
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3124-8_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3123-1, 978-1-4939-3124-8
|
Authors |
Shoichiro Ono |
Abstract |
Dynamics of actin filaments are regulated by a number of actin-binding proteins. To understand the function of an actin-binding protein, it is necessary to characterize effects of the protein on actin filament dynamics in vitro. This chapter describes basic microscopic methods to visualize fluorescently labeled actin filaments using commonly available fluorescence microscope settings. Direct microscopic observation of actin filaments provides strong evidence for severing or bundling of actin filaments. |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 63 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 2 | 3% |
Researcher | 2 | 3% |
Student > Ph. D. Student | 1 | 2% |
Student > Master | 1 | 2% |
Student > Postgraduate | 1 | 2% |
Other | 0 | 0% |
Unknown | 56 | 89% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 10% |
Agricultural and Biological Sciences | 1 | 2% |
Unknown | 56 | 89% |