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Cytoskeleton Methods and Protocols

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Cover of 'Cytoskeleton Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration.
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    Chapter 2 Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast.
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    Chapter 3 Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.
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    Chapter 4 Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.
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    Chapter 5 Imaging Cytoskeleton Components by Electron Microscopy
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    Chapter 6 Purification and Localization of Intraflagellar Transport Particles and Polypeptides
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    Chapter 7 Fluorescence Imaging of the Cytoskeleton in Plant Roots.
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    Chapter 8 Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging
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    Chapter 9 Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament Severing and Bundling
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    Chapter 10 An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Cells
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    Chapter 11 Reconstitution of a Minimal Actin Cortex by Coupling Actin Filaments to Reconstituted Membranes.
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    Chapter 12 Use of Nanobodies to Localize Endogenous Cytoskeletal Proteins and to Determine Their Contribution to Cancer Cell Invasion by Using an ECM Degradation Assay
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    Chapter 13 Actin-Dynamics in Plant Cells: The Function of Actin-Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.
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    Chapter 14 Quantitative Motion Analysis in Two and Three Dimensions
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    Chapter 15 Measurement of Cell Motility Using Microgrooved Substrates
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    Chapter 16 The Study of Cell Motility by Cell Traction Force Microscopy (CTFM)
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    Chapter 17 Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells
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    Chapter 18 Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.
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    Chapter 19 Chemotaxis: Under Agarose Assay
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    Chapter 20 Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.
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    Chapter 21 Cytoskeleton Methods and Protocols
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    Chapter 22 Using a Handheld Gene Gun for Genetic Transformation of Tetrahymena thermophila
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    Chapter 23 Proteomic Tools for the Analysis of Cytoskeleton Proteins.
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    Chapter 24 Homology Modeling Procedures for Cytoskeletal Proteins of Tetrahymena and Other Ciliated Protists.
Attention for Chapter 4: Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.
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Chapter title
Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.
Chapter number 4
Book title
Cytoskeleton Methods and Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3124-8_4
Pubmed ID
Book ISBNs
978-1-4939-3123-1, 978-1-4939-3124-8
Authors

Applewhite, Derek A, Davis, Christine A, Griffis, Eric R, Quintero, Omar A, Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, Omar A. Quintero

Abstract

In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.

X Demographics

X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 20%
Student > Ph. D. Student 1 20%
Researcher 1 20%
Unknown 2 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 20%
Computer Science 1 20%
Agricultural and Biological Sciences 1 20%
Unknown 2 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2021.
All research outputs
#8,070,486
of 24,935,186 outputs
Outputs from Methods in molecular biology
#2,429
of 14,004 outputs
Outputs of similar age
#122,860
of 405,266 outputs
Outputs of similar age from Methods in molecular biology
#268
of 1,463 outputs
Altmetric has tracked 24,935,186 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 14,004 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 405,266 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 1,463 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.