| Chapter title |
Chemotaxis: Under Agarose Assay
|
|---|---|
| Chapter number | 19 |
| Book title |
Cytoskeleton Methods and Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3124-8_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3123-1, 978-1-4939-3124-8
|
| Authors |
Derrick Brazill |
| Abstract |
The unicellular eukaryote Dictyostelium discoideum represents a superb model for examining chemotaxis. Under vegetative conditions, the amoebae are chemotactically responsive to pterins, such as folate. Under starved conditions, they lose their sensitivity to pterins, and become chemotactically responsive to cAMP. As an NIH model system, Dictyostelium offers a variety of advantages in studying chemotaxis, including its conservation of mammalian signaling pathways, its ease of growth, and its genetic tractability. In this chapter, we describe the use of the under agarose chemotaxis assay to identify proteins involved in controlling motility and directional sensing in Dictyostelium discoideum. Given the similarities between Dictyostelium and mammalian cells, this allows us to dissect the conserved pathways involved in eukaryotic chemotaxis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 50% |
| Student > Ph. D. Student | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Unknown | 1 | 25% |