Chapter title |
Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.
|
---|---|
Chapter number | 20 |
Book title |
Cytoskeleton Methods and Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3124-8_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3123-1, 978-1-4939-3124-8
|
Authors |
He, Qi, Roblodowski, Christopher, Qi He, Christopher Roblodowski |
Abstract |
Using Drosophila actin-binding protein Dunc-115 as model system, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single-cell-level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton. |
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