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Cytoskeleton Methods and Protocols

Overview of attention for book
Cover of 'Cytoskeleton Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration.
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    Chapter 2 Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast.
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    Chapter 3 Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.
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    Chapter 4 Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.
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    Chapter 5 Imaging Cytoskeleton Components by Electron Microscopy
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    Chapter 6 Purification and Localization of Intraflagellar Transport Particles and Polypeptides
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    Chapter 7 Fluorescence Imaging of the Cytoskeleton in Plant Roots.
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    Chapter 8 Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging
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    Chapter 9 Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament Severing and Bundling
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    Chapter 10 An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Cells
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    Chapter 11 Reconstitution of a Minimal Actin Cortex by Coupling Actin Filaments to Reconstituted Membranes.
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    Chapter 12 Use of Nanobodies to Localize Endogenous Cytoskeletal Proteins and to Determine Their Contribution to Cancer Cell Invasion by Using an ECM Degradation Assay
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    Chapter 13 Actin-Dynamics in Plant Cells: The Function of Actin-Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.
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    Chapter 14 Quantitative Motion Analysis in Two and Three Dimensions
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    Chapter 15 Measurement of Cell Motility Using Microgrooved Substrates
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    Chapter 16 The Study of Cell Motility by Cell Traction Force Microscopy (CTFM)
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    Chapter 17 Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells
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    Chapter 18 Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.
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    Chapter 19 Chemotaxis: Under Agarose Assay
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    Chapter 20 Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.
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    Chapter 21 Cytoskeleton Methods and Protocols
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    Chapter 22 Using a Handheld Gene Gun for Genetic Transformation of Tetrahymena thermophila
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    Chapter 23 Proteomic Tools for the Analysis of Cytoskeleton Proteins.
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    Chapter 24 Homology Modeling Procedures for Cytoskeletal Proteins of Tetrahymena and Other Ciliated Protists.
Attention for Chapter 10: An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Cells
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Chapter title
An In Vitro Model System to Test Mechano-microbiological Interactions Between Bacteria and Host Cells
Chapter number 10
Book title
Cytoskeleton Methods and Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3124-8_10
Pubmed ID
26498786
Book ISBNs
978-1-4939-3123-1, 978-1-4939-3124-8
Authors

Luís Carlos Santos, Emilia Laura Munteanu, Nicolas Biais

Abstract

The aim of this chapter is to present an innovative technique to visualize changes of the f-actin cytoskeleton in response to locally applied force. We developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated with the Neisseria gonorrhoeae Type IV pili in the same order of magnitude than the forces generated by live bacteria. We saw quick and robust f-actin accumulation at the sites where pulling forces were applied. Using the magnetic tweezers we were able to mimic the local response of the f-actin cytoskeleton to bacteria-generated forces. In this chapter we describe our magnetic tweezers system and show how to control it in order to study cellular responses to force.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Student > Postgraduate 2 25%
Professor > Associate Professor 1 13%
Student > Doctoral Student 1 13%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Agricultural and Biological Sciences 1 13%
Physics and Astronomy 1 13%
Medicine and Dentistry 1 13%
Engineering 1 13%
Other 0 0%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2021.
All research outputs
#15,505,836
of 23,043,346 outputs
Outputs from Methods in molecular biology
#5,403
of 13,194 outputs
Outputs of similar age
#232,264
of 394,754 outputs
Outputs of similar age from Methods in molecular biology
#546
of 1,471 outputs
Altmetric has tracked 23,043,346 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,194 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.

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