Chapter title |
Simultaneous Quantitation of Lamotrigine, Levetiracetam, 10-Hydroxycarbazepine, Topiramate, and Zonisamide in Serum Using HPLC-MS/MS
|
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Chapter number | 4 |
Book title |
Clinical Applications of Mass Spectrometry in Drug Analysis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3252-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3251-1, 978-1-4939-3252-8
|
Authors |
Dean C. Carlow, Heng Shi, Ryan C. Schofield |
Abstract |
Antiepileptic drugs (AEDs) are a diverse group of pharmacological agents used in the treatment of epileptic seizures. Over the past several decades some new AEDs, including lamotrigine (LTG), levetiracetam (LVA), oxcarbazepine (OXC), topiramate (TOP), and zonisamide (ZNS), have become widely used. This chapter describes a very simple and rapid liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of LVA, ZNS, LTG, TOP, and MHD in human serum. The method requires a very small amount of serum (50 μL) for multiple drug measurements and has a total analysis time of 4 min that makes it well suited for routine clinical analysis of several drugs simultaneously. The imprecision (CVs) measured at various concentrations across the analytical measurement range (AMR) are less than 7 % for all analytes. The AMR for each analyte is as follows: LVA (1-100 μg/mL), ZNS (0.8-80 μg/mL), TOP (0.5-50 μg/mL), and 0.6-60 μg/mL for LTG and MHD. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 19% |
Student > Doctoral Student | 3 | 19% |
Professor > Associate Professor | 3 | 19% |
Student > Postgraduate | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Other | 3 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 25% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 19% |
Chemistry | 2 | 13% |
Neuroscience | 2 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 25% |