| Chapter title |
A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan
|
|---|---|
| Chapter number | 9 |
| Book title |
Clinical Applications of Mass Spectrometry in Drug Analysis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3252-8_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3251-1, 978-1-4939-3252-8
|
| Authors |
Shuang Deng, Michael Kiscoan, Clint Frazee, Susan Abdel-Rahman, Jignesh Dalal, Uttam Garg |
| Abstract |
Busulfan is an alkylating agent widely used in the ablation of bone marrow cells before hematopoietic stem cell transplant. Due to large intraindividual and interindividual variations, and narrow therapeutic window, therapeutic drug monitoring of busulfan is warranted. A quick and reliable HPLC-MS/MS method was developed for the assay of plasma busulfan. HPLC involved C18 column, and MS/MS was used in electrospray ionization (ESI) positive mode. Quantitation and identification of busulfan was made using various multiple reactions monitoring (MRMs). Isotopic labeled busulfan-d8 was used as the internal standard. The method is linear from 50 to 2500 ng/mL and has with-in run and between-run imprecision of <10 %. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Student > Postgraduate | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 33% |
| Agricultural and Biological Sciences | 1 | 17% |
| Neuroscience | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |
| Unknown | 1 | 17% |