Chapter title |
Detection of Stimulants and Narcotics by Liquid Chromatography-Tandem Mass Spectrometry and Gas Chromatography-Mass Spectrometry for Sports Doping Control
|
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Chapter number | 26 |
Book title |
Clinical Applications of Mass Spectrometry in Drug Analysis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3252-8_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3251-1, 978-1-4939-3252-8
|
Authors |
Brian D. Ahrens, Yulia Kucherova, Anthony W. Butch, Ahrens, Brian D., Kucherova, Yulia, Butch, Anthony W. |
Abstract |
Sports drug testing laboratories are required to detect several classes of compounds that are prohibited at all times, which include anabolic agents, peptide hormones, growth factors, beta-2 agonists, hormones and metabolic modulators, and diuretics/masking agents. Other classes of compounds such as stimulants, narcotics, cannabinoids, and glucocorticoids are also prohibited, but only when an athlete is in competition. A single class of compounds can contain a large number of prohibited substances and all of the compounds should be detected by the testing procedure. Since there are almost 70 stimulants on the prohibited list it can be a challenge to develop a single screening method that will optimally detect all the compounds. We describe a combined liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) testing method for detection of all the stimulants and narcotics on the World Anti-Doping Agency prohibited list. Urine for LC-MS/MS testing does not require sample pretreatment and is a direct dilute and shoot method. Urine samples for the GC-MS method require a liquid-liquid extraction followed by derivatization with trifluoroacetic anhydride. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 31 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 13% |
Student > Ph. D. Student | 4 | 13% |
Researcher | 2 | 6% |
Other | 2 | 6% |
Lecturer | 1 | 3% |
Other | 3 | 10% |
Unknown | 15 | 48% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 13% |
Agricultural and Biological Sciences | 3 | 10% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Sports and Recreations | 2 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 3 | 10% |
Unknown | 16 | 52% |