| Chapter title |
Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS)
|
|---|---|
| Chapter number | 3 |
| Book title |
Clinical Applications of Mass Spectrometry in Drug Analysis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3252-8_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3251-1, 978-1-4939-3252-8
|
| Authors |
Jaime H. Noguez, James C. Ritchie, Noguez, Jaime H., Ritchie, James C. |
| Abstract |
This chapter describes a method to measure the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma samples using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The instrument is operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source in positive ionization mode. Samples are extracted with a 90:10 methanol/0.1 N hydrochloric acid solution containing stable isotope-labeled internal standards for each analyte. After centrifugation the supernatant is transferred to a mass spectrometry vial, injected onto the UPLC-ESI-MS/MS, and quantified using an eight-point calibration curve. |
Mendeley readers
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| Other | 0 | 0% |
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