Chapter title |
Quantification of Docetaxel in Serum Using Turbulent Flow Liquid Chromatography Electrospray Tandem Mass Spectrometry (TFC-HPLC-ESI-MS/MS)
|
---|---|
Chapter number | 14 |
Book title |
Clinical Applications of Mass Spectrometry in Drug Analysis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3252-8_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3251-1, 978-1-4939-3252-8
|
Authors |
Christopher A. Crutchfield, Mark A. Marzinke, William A. Clarke |
Abstract |
Docetaxel is a second-generation taxane and is used clinically as an anti-neoplastic agent in cancer chemotherapy via an anti-mitotic mechanism. Its efficacy is limited to a narrow therapeutic window. Inappropriately high concentrations may cause erythema, fluid retention, nausea, diarrhea, and neutropenia. As a result, dosing recommendations have changed from high dosage loading every 3 weeks to lower dosage loading weekly. We describe a method that can be used for therapeutic drug monitoring of docetaxel levels using turbulent flow liquid chromatography electrospray tandem mass spectrometry (TFC-HPLC-ESI-MS/MS). The method is rapid, requiring only 6.3 min per analytical run following a simple protein crash. The method requires only 100 μL of serum. Concentrations of docetaxel were quantified by a calibration curve relating the peak-area ratio of docetaxel to a deuterated internal standard (docetaxel-D9). The method was linear from 7.8 to 1000 ng/mL, with imprecision ≤6.2 %. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 13% |
Unknown | 7 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 3 | 38% |
Student > Doctoral Student | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Student > Master | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 25% |
Chemistry | 2 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Neuroscience | 1 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |