Chapter title |
Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions
|
---|---|
Chapter number | 15 |
Book title |
Telomeres and Telomerase
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6892-3_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6891-6, 978-1-4939-6892-3
|
Authors |
Wenbin Ma, Ok-hee Lee, Hyeung Kim, Zhou Songyang |
Editors |
Zhou Songyang |
Abstract |
Studying protein-protein interactions is critical to our understanding of signaling pathways. Telomere Interactome is assembled around telomeres and consists of proteins and factors from diverse pathways. Dissecting how this protein network contributes to telomere protection and length regulation requires the elucidation of the complex and dynamic interactions between the proteins within the interactome. Here, we focus on three assays, Bi-molecular Fluorescence Complementation (BiFC), Gaussia Luciferase Protein-fragment Complementation Assay (GLuc PCA), and OPAL peptide array, which have proven vital in our studies of telomere protein interaction networks. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 50% |
Student > Doctoral Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 50% |
Unknown | 2 | 50% |