Chapter title |
Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9
|
---|---|
Chapter number | 2 |
Book title |
Telomeres and Telomerase
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6892-3_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6891-6, 978-1-4939-6892-3
|
Authors |
Jun Xu, Zhou Songyang, Dan Liu, Hyeung Kim, Xu, Jun, Songyang, Zhou, Liu, Dan, Kim, Hyeung |
Editors |
Zhou Songyang |
Abstract |
Telomeres play an important role in ensuring the integrity of the genome. Telomere shortening can lead to loss of genetic information and trigger DNA damage responses. Cultured mammalian cells have served as critical model systems for studying the function of telomere binding proteins and telomerase. Tremendous heterogeneity can be observed both between species and within a single cell population. Recent advances in genome editing (such as the development of the CRISPR/Cas9 platform) have further enabled researchers to carry out loss-of-function analysis of how disrupting key players in telomere maintenance affects telomere length regulation. Here we describe the steps to be carried out in order to analyze the average length of telomeres in CRISPR-engineered human knockout (KO) cells (TRF analysis). |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 13% |
Other | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 5 | 63% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Engineering | 1 | 13% |
Unknown | 3 | 38% |