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Mendeley readers
Chapter title |
Telomere G-Rich Overhang Length Measurement: DSN Method
|
---|---|
Chapter number | 5 |
Book title |
Telomeres and Telomerase
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6892-3_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6891-6, 978-1-4939-6892-3
|
Authors |
Yong Zhao, Jerry W. Shay, Woodring E. Wright |
Editors |
Zhou Songyang |
Abstract |
Telomeres terminate in 3' single-stranded G-rich overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex specific nuclease (DSN) to digest all double-stranded DNA including telomeres to fragments of <10 bp, leaving the single-stranded overhangs intact. Their length can then be determined by Southern blot using super-sensitive telomere C-rich probes. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 33% |
Professor | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Researcher | 1 | 17% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 2 | 33% |
Medicine and Dentistry | 2 | 33% |