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Telomeres and Telomerase

Overview of attention for book
Telomeres and Telomerase
Springer New York

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to Telomeres and Telomerase
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    Chapter 2 Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9
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    Chapter 3 Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH)
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    Chapter 4 Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)
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    Chapter 5 Telomere G-Rich Overhang Length Measurement: DSN Method
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    Chapter 6 Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA
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    Chapter 7 Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
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    Chapter 8 Analysis of Yeast Telomerase by Primer Extension Assays
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    Chapter 9 Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP)
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    Chapter 10 Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™)
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    Chapter 11 Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques
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    Chapter 12 Cytogenetic Analysis of Telomere Dysfunction
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    Chapter 13 Probing the Telomere Damage Response
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    Chapter 14 Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins
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    Chapter 15 Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions
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    Chapter 16 In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits
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    Chapter 17 Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
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    Chapter 18 Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization
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    Chapter 19 Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
Attention for Chapter 18: Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization
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Chapter title
Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization
Chapter number 18
Book title
Telomeres and Telomerase
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6892-3_18
Pubmed ID
28324510
Book ISBNs
978-1-4939-6891-6, 978-1-4939-6892-3
Authors

Zepeng Zhang, Qian Hu, Yong Zhao

Editors

Zhou Songyang

Abstract

In mammalian cells, in addition to double-stranded telomeric DNA at chromosome ends, extra telomere-homologous DNA is present that adopts different conformations, including single-stranded G- or C-rich DNA, extrachromosomal circular DNA (T-circle), and telomeric complex (T-complex) with an unidentified structure. The formation of such telomere-homologous DNA is closely related to telomeric DNA metabolism and chromosome end protection by telomeres. Conventional agarose gel electrophoresis is unable to separate DNA based on conformation. Here, we introduce the method of two-dimensional (2D) agarose electrophoresis in combination with in-gel native/denatured hybridization to determine different conformations formed by telomere-homologous DNA.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 60%
Student > Doctoral Student 1 20%
Student > Master 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 60%
Materials Science 1 20%
Medicine and Dentistry 1 20%

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