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Mendeley readers
| Chapter title |
Detection of Protein Interactions in T3S Systems Using Yeast Two-Hybrid Analysis.
|
|---|---|
| Chapter number | 19 |
| Book title |
Type 3 Secretion Systems
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6649-3_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
| Authors |
Matthew L. Nilles |
| Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
| Abstract |
Two-hybrid systems, sometimes termed interaction traps, are genetic systems designed to find and analyze interactions between proteins. The most common systems are yeast based (commonly Saccharomyces cerevisae) and rely on the functional reconstitution of the GAL4 transcriptional activator. Reporter genes, such as the lacZ gene of Escherichia coli (encodes β-galactosidase), are placed under GAL4-dependent transcriptional control to provide quick and reliable detection of protein interactions. In this method the use of a yeast-based two-hybrid system is described to study protein interactions between components of type III secretion systems. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 100% |