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Chapter title |
Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.
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---|---|
Chapter number | 16 |
Book title |
Type 3 Secretion Systems
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6649-3_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
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Authors |
Travis D. Alvine, Patrick Osei-Owusu, Danielle L. Jessen Condry, Matthew L. Nilles |
Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
Abstract |
The ability to express and purify recombinant needle proteins from the Type III Secretion System (T3SS) of many gram-negative bacteria has allowed us to develop novel experimental approaches, both in vitro and in vivo, to identify unique roles for T3SS in bacterial pathogenesis. In addition, these purified needle proteins have shown to be promising immunotherapies acting as both protective antigens and adjuvants, presumably due to their immune activating properties. Here, we describe the expression and purification of recombinant T3SS needle proteins. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 20% |
Researcher | 1 | 20% |
Unknown | 3 | 60% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 40% |
Unknown | 3 | 60% |