| Chapter title |
Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.
|
|---|---|
| Chapter number | 7 |
| Book title |
Type 3 Secretion Systems
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6649-3_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
| Authors |
Philip R. Adam, Michael L. Barta, Nicholas E. Dickenson |
| Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
| Abstract |
In vitro characterization of type III secretion system (T3SS) translocator proteins has proven challenging due to complex purification schemes and their hydrophobic nature that often requires detergents to provide protein solubility and stability. Here, we provide experimental details for several techniques that overcome these hurdles, allowing for the direct characterization of the Shigella translocator protein IpaB with respect to phospholipid membrane interaction. The techniques specifically discussed in this chapter include membrane interaction/liposome flotation, liposome sensitive fluorescence quenching, and protein-mediated liposome disruption assays. These assays have provided valuable insight into the role of IpaB in T3SS-mediated phospholipid membrane interactions by Shigella and should readily extend to other members of this important class of proteins. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Nursing and Health Professions | 1 | 100% |