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Type 3 Secretion Systems

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Cover of 'Type 3 Secretion Systems'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to Type III Secretion Systems.
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    Chapter 2 Site-Directed Mutagenesis and Its Application in Studying the Interactions of T3S Components.
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    Chapter 3 Blue Native Protein Electrophoresis to Study the T3S System Using Yersinia pestis as a Model.
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    Chapter 4 In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated Using the Yersinia pestis T3S System.
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    Chapter 5 Isolation of Type III Secretion System Needle Complexes by Shearing.
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    Chapter 6 Use of Transcriptional Control to Increase Secretion of Heterologous Proteins in T3S Systems.
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    Chapter 7 Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.
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    Chapter 8 Analysis of Type III Secretion System Secreted Proteins.
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    Chapter 9 Fractionation Techniques to Examine Effector Translocation.
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    Chapter 10 Measurement of Effector Protein Translocation Using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.
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    Chapter 11 A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas.
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    Chapter 12 Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants.
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    Chapter 13 A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants.
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    Chapter 14 Detecting Immune Responses to Type III Secretion Systems.
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    Chapter 15 Recombinant Expression and Purification of the Shigella Translocator IpaB.
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    Chapter 16 Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.
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    Chapter 17 Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to These Proteins.
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    Chapter 18 Identification of the Targets of Type III Secretion System Inhibitors.
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    Chapter 19 Detection of Protein Interactions in T3S Systems Using Yeast Two-Hybrid Analysis.
Attention for Chapter 1: Introduction to Type III Secretion Systems.
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Chapter title
Introduction to Type III Secretion Systems.
Chapter number 1
Book title
Type 3 Secretion Systems
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6649-3_1
Pubmed ID
Book ISBNs
978-1-4939-6647-9, 978-1-4939-6649-3
Authors

Danielle L. Jessen Condry, Matthew L. Nilles

Editors

Matthew L. Nilles, Danielle L. Jessen Condry

Abstract

Type III secretion (T3S) systems are found in a large number of gram-negative bacteria where they function to manipulate the biology of infected hosts. Hosts targeted by T3S systems are widely distributed in nature and are represented by animals and plants. T3S systems are found in diverse genera of bacteria and they share a common core structure and function. Effector proteins are delivered by T3S systems into targeted host cells without prior secretion of the effectors into the environment. Instead, an assembled translocon structure functions to translocate effectors across eukaryotic cell membranes. In many cases, T3S systems are essential virulence factors and in some instances they promote symbiotic interactions.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 25%
Student > Bachelor 2 25%
Student > Ph. D. Student 1 13%
Unspecified 1 13%
Unknown 2 25%
Readers by discipline Count As %
Unspecified 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Nursing and Health Professions 1 13%
Agricultural and Biological Sciences 1 13%
Computer Science 1 13%
Other 1 13%
Unknown 2 25%