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Mendeley readers
| Chapter title |
Recombinant Expression and Purification of the Shigella Translocator IpaB.
|
|---|---|
| Chapter number | 15 |
| Book title |
Type 3 Secretion Systems
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6649-3_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
| Authors |
Michael L. Barta, Philip R. Adam, Nicholas E. Dickenson |
| Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
| Abstract |
Type III secretion systems (T3SS) are highly conserved virulence factors employed by a large number of pathogenic gram-negative bacteria. Like many T3SS translocators, recombinant expression of the hydrophobic Shigella protein IpaB requires the presence of its cognate chaperone IpgC. Chaperone-bound IpaB is maintained in a nonfunctional state, which has hampered in vitro studies aimed at understanding molecular structure and function of this important class of T3SS proteins. Herein, we describe an expression and purification protocol that utilizes mild detergents to produce highly purified, homogeneous IpaB of defined oligomeric states. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 33% |
| Professor > Associate Professor | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Chemical Engineering | 1 | 17% |
| Unknown | 3 | 50% |