| Chapter title |
Detecting Immune Responses to Type III Secretion Systems.
|
|---|---|
| Chapter number | 14 |
| Book title |
Type 3 Secretion Systems
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6649-3_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
| Authors |
Peter L. Knopick, David S. Bradley |
| Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
| Abstract |
Measuring immune responses against type III secretion and related molecules has been made easier with the advent of reporter cell lines. For example, the THP-1-XBlue and HEK-Blue cells from InvivoGen provide easy detection of gene activation under NF-kB and AP-1 control. In addition, many of these cells have been engineered to express specific pattern recognition receptors (PRRs) helping to elucidate activation pathways. In addition, the THP-1 parent cell line from ATCC provides a useful tool for evaluating cytokine release upon stimulation. This chapter will outline using X-blue reporter cell lines, setting up experiments to measure cytokine secretion, and general guidelines for cytokine quantification. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 100% |