| Chapter title |
Site-Directed Mutagenesis and Its Application in Studying the Interactions of T3S Components.
|
|---|---|
| Chapter number | 2 |
| Book title |
Type 3 Secretion Systems
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6649-3_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
| Authors |
Matthew S. Francis, Ayad A. A. Amer, Debra L. Milton, Tiago R. D. Costa |
| Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
| Abstract |
Type III secretion systems are a prolific virulence determinant among Gram-negative bacteria. They are used to paralyze the host cell, which enables bacterial pathogens to establish often fatal infections-unless an effective therapeutic intervention is available. However, as a result of a catastrophic rise in infectious bacteria resistant to conventional antibiotics, these bacteria are again a leading cause of worldwide mortality. Hence, this report describes a pDM4-based site-directed mutagenesis strategy that is assisting in our foremost objective to better understand the fundamental workings of the T3SS, using Yersinia as a model pathogenic bacterium. Examples are given that clearly document how pDM4-mediated site-directed mutagenesis has been used to establish clean point mutations and in-frame deletion mutations that have been instrumental in identifying and understanding the molecular interactions between components of the Yersinia type III secretion system. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 20% |
| Researcher | 4 | 20% |
| Student > Bachelor | 3 | 15% |
| Other | 1 | 5% |
| Professor | 1 | 5% |
| Other | 3 | 15% |
| Unknown | 4 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 35% |
| Nursing and Health Professions | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Unspecified | 1 | 5% |
| Arts and Humanities | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 30% |