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Chapter title |
Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to These Proteins.
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Chapter number | 17 |
Book title |
Type 3 Secretion Systems
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6649-3_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
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Authors |
Travis D. Alvine, David S. Bradley, Matthew L. Nilles |
Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
Abstract |
Many Gram-negative pathogens utilize a type III secretion (T3S) system to directly deliver effector molecules into host eukaryotic cells to manipulate cellular processes. These surface-exposed syringe-like structures are highly conserved, necessary for pathogenesis, and hence are therapeutic targets against a number of Gram-negative pathogens. Here we describe a protocol for using purified needle proteins to immunize mice, and subsequently, ways to characterize the immune response to immunization. |