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Mendeley readers
| Chapter title |
Measurement of Effector Protein Translocation Using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.
|
|---|---|
| Chapter number | 10 |
| Book title |
Type 3 Secretion Systems
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6649-3_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
| Authors |
Sara Schesser Bartra, Gregory V. Plano |
| Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
| Abstract |
Numerous bacterial pathogens employ specialized protein secretion machineries to directly inject anti-host proteins, termed effector proteins, into eukaryotic cells. Effector proteins carrying small phosphorylatable tags can be used to detect and quantify effector protein injection. Here, we describe the use of the ELK- and GSK-tags to detect the translocation of the Y. pestis YopE effector protein into RAW 264.7 macrophage-like cells using immunoblot analysis with phospho-specific antibodies. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 38% |
| Unspecified | 1 | 13% |
| Librarian | 1 | 13% |
| Professor | 1 | 13% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 25% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Unspecified | 1 | 13% |
| Computer Science | 1 | 13% |
| Nursing and Health Professions | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |