Chapter title |
Scrape Loading/Dye Transfer Assay.
|
---|---|
Chapter number | 9 |
Book title |
Gap Junction Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3664-9_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
Authors |
Pavel Babica, Iva Sovadinová, Brad L. Upham |
Editors |
Mathieu Vinken, Scott R. Johnstone |
Abstract |
The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs. |
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