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Gap Junction Protocols

Overview of attention for book
Cover of 'Gap Junction Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.
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    Chapter 2 Gap Junction Protocols
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    Chapter 3 Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.
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    Chapter 4 Immunohisto- and Cytochemistry Analysis of Connexins.
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    Chapter 5 Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.
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    Chapter 6 Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor.
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    Chapter 7 Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells.
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    Chapter 8 Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis.
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    Chapter 9 Scrape Loading/Dye Transfer Assay.
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    Chapter 10 Microinjection Technique for Assessment of Gap Junction Function.
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    Chapter 11 Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.
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    Chapter 12 Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
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    Chapter 13 Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging.
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    Chapter 14 Gap Junction Protocols
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    Chapter 15 Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels.
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    Chapter 16 Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
Attention for Chapter 5: Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.
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  • Good Attention Score compared to outputs of the same age and source (71st percentile)

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Chapter title
Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.
Chapter number 5
Book title
Gap Junction Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3664-9_5
Pubmed ID
27207287
Book ISBNs
978-1-4939-3662-5, 978-1-4939-3664-9
Authors

Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, Brant E. Isakson, Good, Miranda E, Begandt, Daniela, DeLalio, Leon J, Johnstone, Scott R, Isakson, Brant E, Good, Miranda E., DeLalio, Leon J., Johnstone, Scott R., Isakson, Brant E.

Editors

Mathieu Vinken, Scott R. Johnstone

Abstract

Global knockout of vascular connexins can result in premature/neonatal death, severe developmental complications, or compensatory up-regulation of different connexin isoforms. Thus, specific connexin gene knockdown using RNAi-mediated technologies is a technique that allows investigators to efficiently monitor silencing effects of single or multiple connexin gene products. The present chapter describes the transient knockdown of connexins in vitro and ex vivo for cells of the blood vessel wall. In detail, different transfection methods for primary endothelial cells and ex vivo thoracodorsal arteries are described. Essential controls for validating transfection efficiency as well as targeted gene knockdown are explained. These protocols provide researchers with the ability to modify connexin gene expression levels in a multitude of experimental setups.

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 25%
Professor > Associate Professor 1 25%
Student > Bachelor 1 25%
Researcher 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Agricultural and Biological Sciences 1 25%
Medicine and Dentistry 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
All research outputs
#13,985,864
of 22,881,154 outputs
Outputs from Methods in molecular biology
#3,940
of 13,133 outputs
Outputs of similar age
#199,674
of 393,699 outputs
Outputs of similar age from Methods in molecular biology
#386
of 1,471 outputs
Altmetric has tracked 22,881,154 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,133 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,699 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.

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