You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Scrape Loading/Dye Transfer Assay.
|
|---|---|
| Chapter number | 9 |
| Book title |
Gap Junction Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3664-9_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
| Authors |
Pavel Babica, Iva Sovadinová, Brad L. Upham |
| Editors |
Mathieu Vinken, Scott R. Johnstone |
| Abstract |
The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs. |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 20% |
| Researcher | 8 | 20% |
| Student > Doctoral Student | 4 | 10% |
| Student > Master | 4 | 10% |
| Student > Bachelor | 3 | 7% |
| Other | 2 | 5% |
| Unknown | 12 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 17% |
| Biochemistry, Genetics and Molecular Biology | 6 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 12% |
| Medicine and Dentistry | 2 | 5% |
| Environmental Science | 2 | 5% |
| Other | 7 | 17% |
| Unknown | 12 | 29% |