| Chapter title |
Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.
|
|---|---|
| Chapter number | 3 |
| Book title |
Gap Junction Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3664-9_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
| Authors |
Joost Willebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, Mathieu Vinken |
| Editors |
Mathieu Vinken, Scott R. Johnstone |
| Abstract |
Since connexin expression is partly regulated at the protein level, immunoblot analysis represents a frequently addressed technique in the connexin research field. The present chapter describes the setup of an immunoblot procedure, including protein extraction and quantification from biological samples, gel electrophoresis, protein transfer, and immunoblotting, which is optimized for analysis of connexins in liver tissue. In essence, proteins are separated on a polyacrylamide gel using sodium dodecyl sulfate followed by transfer of proteins on a nitrocellulose membrane. The latter allows specific detection of connexins with antibodies combined with revelation through enhanced chemiluminescence. |
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