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Gap Junction Protocols

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Cover of 'Gap Junction Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.
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    Chapter 2 Gap Junction Protocols
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    Chapter 3 Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.
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    Chapter 4 Immunohisto- and Cytochemistry Analysis of Connexins.
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    Chapter 5 Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.
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    Chapter 6 Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor.
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    Chapter 7 Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells.
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    Chapter 8 Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis.
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    Chapter 9 Scrape Loading/Dye Transfer Assay.
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    Chapter 10 Microinjection Technique for Assessment of Gap Junction Function.
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    Chapter 11 Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.
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    Chapter 12 Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
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    Chapter 13 Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging.
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    Chapter 14 Gap Junction Protocols
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    Chapter 15 Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels.
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    Chapter 16 Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
Attention for Chapter 2: Gap Junction Protocols
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Chapter title
Gap Junction Protocols
Chapter number 2
Book title
Gap Junction Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3664-9_2
Pubmed ID
Book ISBNs
978-1-4939-3662-5, 978-1-4939-3664-9
Authors

Liu, Xiaoming, Xu, Canxia, Xiaoming Liu, Canxia Xu

Editors

Mathieu Vinken, Scott R. Johnstone

Abstract

Connexins are the structural proteins of gap junctions and their functioning as tumor suppressors is well known. Epigenetic modifications, such as methylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in human gastric tissues using methylation-specific PCR, bisulfite-specific PCR sequencing as well as MassArray techniques.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Lecturer > Senior Lecturer 1 33%
Unknown 1 33%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 33%
Medicine and Dentistry 1 33%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2016.
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#20,328,845
of 22,873,031 outputs
Outputs from Methods in molecular biology
#9,915
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Outputs of similar age
#330,715
of 393,675 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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So far Altmetric has tracked 13,130 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.