Chapter title |
Immunohisto- and Cytochemistry Analysis of Connexins.
|
---|---|
Chapter number | 4 |
Book title |
Gap Junction Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3664-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
Authors |
Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina Da Silva, Sara Crespo Yanguas, Mathieu Vinken |
Editors |
Mathieu Vinken, Scott R. Johnstone |
Abstract |
Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing, and/or protein expression level. Basically, fluorescent IHC is a short, simple, and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition. |
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