| Chapter title |
Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
|
|---|---|
| Chapter number | 12 |
| Book title |
Gap Junction Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3664-9_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
| Authors |
Maria Kuzma-Kuzniarska, Clarence Yapp, Philippa A. Hulley, Kuzma-Kuzniarska, Maria, Yapp, Clarence, Hulley, Philippa A. |
| Editors |
Mathieu Vinken, Scott R. Johnstone |
| Abstract |
Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique to study the movement of fluorescent molecules inside a cell. Although initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to perform FRAP experiment to study gap junctional communication in living cells. The procedures described here can be carried out with a laser-scanning confocal microscope and any in vitro cultured cells known to communicate via gap junctions. In addition, the method can be easily adjusted to measure gap junction function in 3D cell cultures as well as ex vivo tissue. |
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