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Gap Junction Protocols

Overview of attention for book
Cover of 'Gap Junction Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.
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    Chapter 2 Gap Junction Protocols
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    Chapter 3 Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.
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    Chapter 4 Immunohisto- and Cytochemistry Analysis of Connexins.
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    Chapter 5 Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.
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    Chapter 6 Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor.
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    Chapter 7 Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells.
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    Chapter 8 Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis.
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    Chapter 9 Scrape Loading/Dye Transfer Assay.
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    Chapter 10 Microinjection Technique for Assessment of Gap Junction Function.
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    Chapter 11 Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.
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    Chapter 12 Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
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    Chapter 13 Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging.
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    Chapter 14 Gap Junction Protocols
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    Chapter 15 Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels.
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    Chapter 16 Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
Attention for Chapter 6: Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor.
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Chapter title
Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor.
Chapter number 6
Book title
Gap Junction Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3664-9_6
Pubmed ID
Book ISBNs
978-1-4939-3662-5, 978-1-4939-3664-9
Authors

Mark Kibschull, Stephen J. Lye, Oksana Shynlova

Editors

Mathieu Vinken, Scott R. Johnstone

Abstract

Transgenic mouse models have demonstrated critical roles for gap junctions in establishing a successful pregnancy. To study the cellular and molecular mechanisms, the use of cell culture systems is essential to discriminate between the effects of different connexin isoforms expressed in individual cells or tissues of the developing conceptus or in maternal reproductive tissues. The generation and analysis of gene-deficient trophoblast stem cell lines from mice clearly revealed the functions of connexins in regulating placental development. This chapter focuses on the use of connexin gene-deficient trophoblast stem cell cultures to reveal the individual role of gap junctions in regulating trophoblast differentiation and proliferation in vitro under controlled conditions. In addition, cultures of primary uterine myocytes, isolated from mice or rats, allow studying the effects of mechanical stretch or ovarian hormones on regulating connexin expression, and thus, to model the molecular mechanisms of uterine growth and development during pregnancy. Here, we describe the derivation of primary uterine myocyte cultures and their use in in vitro stretch experiments to study the mechanisms of myometrial remodeling essential to accommodate the growing fetus throughout gestation.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 33%
Researcher 1 17%
Student > Doctoral Student 1 17%
Student > Master 1 17%
Unknown 1 17%
Readers by discipline Count As %
Medicine and Dentistry 3 50%
Immunology and Microbiology 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
All research outputs
#15,380,359
of 22,881,154 outputs
Outputs from Methods in molecular biology
#5,350
of 13,133 outputs
Outputs of similar age
#230,984
of 393,699 outputs
Outputs of similar age from Methods in molecular biology
#545
of 1,471 outputs
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So far Altmetric has tracked 13,133 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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