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Angiogenesis Protocols

Overview of attention for book
Cover of 'Angiogenesis Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Angiogenesis Protocols
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    Chapter 2 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
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    Chapter 3 Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis
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    Chapter 4 Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
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    Chapter 5 Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
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    Chapter 6 Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells
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    Chapter 7 Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
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    Chapter 8 In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis
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    Chapter 9 Tube-Forming Assays
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    Chapter 10 Angiogenesis Protocols
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    Chapter 11 Spheroid-Based In Vitro Angiogenesis Model
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    Chapter 12 Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
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    Chapter 13 Angiogenesis Protocols
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    Chapter 14 A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
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    Chapter 15 Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis
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    Chapter 16 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
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    Chapter 17 Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
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    Chapter 18 Angiogenesis Protocols
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    Chapter 19 Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
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    Chapter 20 Angiogenesis Protocols
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    Chapter 21 Angiogenesis Protocols
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    Chapter 22 Models of Oxygen Induced Retinopathy in Rodents
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    Chapter 23 The Sponge Implant Model of Angiogenesis
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    Chapter 24 Angiogenesis Protocols
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    Chapter 25 Angiogenesis Protocols
  27. Altmetric Badge
    Chapter 26 Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature
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    Chapter 27 Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos
Attention for Chapter 10: Angiogenesis Protocols
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Chapter title
Angiogenesis Protocols
Chapter number 10
Book title
Angiogenesis Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3628-1_10
Pubmed ID
27172952
Book ISBNs
978-1-4939-3626-7, 978-1-4939-3628-1
Authors

Richards, Mark, Mellor, Harry, Mark Richards, Harry Mellor

Abstract

During angiogenesis, endothelial cells invade into the stromal matrix: a complex, structured array of extracellular matrix proteins. This three-dimensional deformable substrate also contains a mixture of angiogenic factors as well as embedded stromal cells. Interactions between endothelial cells and the stromal tissue make complex and important contributions to the process of angiogenesis; however, the composition of the stromal matrix is hard to replicate in vitro. The coculture angiogenesis assay is a long-term assay that uses fibroblasts to secrete and condition a stromal matrix that more closely mimics tissue than a simple collagen gel. Like all in vitro assays of angiogenesis, it has both strengths and weaknesses. Here we give protocols for the two of the most useful applications of the assay: screening for regulators of angiogenesis and high-resolution imaging.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Professor 3 27%
Student > Ph. D. Student 2 18%
Student > Master 2 18%
Student > Bachelor 1 9%
Student > Postgraduate 1 9%
Other 0 0%
Unknown 2 18%
Readers by discipline Count As %
Medicine and Dentistry 3 27%
Biochemistry, Genetics and Molecular Biology 2 18%
Sports and Recreations 1 9%
Materials Science 1 9%
Engineering 1 9%
Other 0 0%
Unknown 3 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2016.
All research outputs
#20,326,948
of 22,870,727 outputs
Outputs from Methods in molecular biology
#9,917
of 13,129 outputs
Outputs of similar age
#330,701
of 393,666 outputs
Outputs of similar age from Methods in molecular biology
#1,053
of 1,470 outputs
Altmetric has tracked 22,870,727 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,129 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,666 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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