↓ Skip to main content

Angiogenesis Protocols

Overview of attention for book
Cover of 'Angiogenesis Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Angiogenesis Protocols
  3. Altmetric Badge
    Chapter 2 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
  4. Altmetric Badge
    Chapter 3 Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis
  5. Altmetric Badge
    Chapter 4 Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
  6. Altmetric Badge
    Chapter 5 Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
  7. Altmetric Badge
    Chapter 6 Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells
  8. Altmetric Badge
    Chapter 7 Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
  9. Altmetric Badge
    Chapter 8 In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis
  10. Altmetric Badge
    Chapter 9 Tube-Forming Assays
  11. Altmetric Badge
    Chapter 10 Angiogenesis Protocols
  12. Altmetric Badge
    Chapter 11 Spheroid-Based In Vitro Angiogenesis Model
  13. Altmetric Badge
    Chapter 12 Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
  14. Altmetric Badge
    Chapter 13 Angiogenesis Protocols
  15. Altmetric Badge
    Chapter 14 A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
  16. Altmetric Badge
    Chapter 15 Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis
  17. Altmetric Badge
    Chapter 16 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
  18. Altmetric Badge
    Chapter 17 Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
  19. Altmetric Badge
    Chapter 18 Angiogenesis Protocols
  20. Altmetric Badge
    Chapter 19 Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
  21. Altmetric Badge
    Chapter 20 Angiogenesis Protocols
  22. Altmetric Badge
    Chapter 21 Angiogenesis Protocols
  23. Altmetric Badge
    Chapter 22 Models of Oxygen Induced Retinopathy in Rodents
  24. Altmetric Badge
    Chapter 23 The Sponge Implant Model of Angiogenesis
  25. Altmetric Badge
    Chapter 24 Angiogenesis Protocols
  26. Altmetric Badge
    Chapter 25 Angiogenesis Protocols
  27. Altmetric Badge
    Chapter 26 Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature
  28. Altmetric Badge
    Chapter 27 Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos
Attention for Chapter 5: Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
Altmetric Badge

Citations

dimensions_citation
7 Dimensions

Readers on

mendeley
20 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
Chapter number 5
Book title
Angiogenesis Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3628-1_5
Pubmed ID
Book ISBNs
978-1-4939-3626-7, 978-1-4939-3628-1
Authors

Zerina Lokmic, Lokmic, Zerina

Abstract

A protocol describing the isolation of foreskin lymphatic endothelial cells (LECs) and lymphatic malformation lymphatic endothelial cells (LM LECs) is presented herein. To isolate LECs and LM LECs, tissues are mechanically disrupted to make a single-cell suspension, which is then enzymatically digested in dispase and collagenase type II. LECs and LM LECs, in the resulting single-cell suspension, are then sequentially labeled with antibodies recognizing fibroblast and endothelial cell surface antigens CD34 and CD31 and separated from the remaining components in the cell suspension by capture with magnetic beads. Viable LECs and LM LECs are then seeded and expanded on fibronectin-coated flasks. LEC and LM LEC purity is determined immunohistochemically using cell surface markers CD31, CD34, podoplanin, VEGFR-3 and nuclear marker PROX-1. Cells whose purity is >98 % are used for experiments between passage 4 and 6.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 15%
Student > Bachelor 3 15%
Student > Postgraduate 3 15%
Student > Doctoral Student 2 10%
Professor 2 10%
Other 4 20%
Unknown 3 15%
Readers by discipline Count As %
Medicine and Dentistry 6 30%
Agricultural and Biological Sciences 4 20%
Biochemistry, Genetics and Molecular Biology 3 15%
Engineering 2 10%
Veterinary Science and Veterinary Medicine 1 5%
Other 2 10%
Unknown 2 10%