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Angiogenesis Protocols

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Cover of 'Angiogenesis Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Angiogenesis Protocols
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    Chapter 2 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
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    Chapter 3 Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis
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    Chapter 4 Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
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    Chapter 5 Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
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    Chapter 6 Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells
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    Chapter 7 Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
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    Chapter 8 In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis
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    Chapter 9 Tube-Forming Assays
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    Chapter 10 Angiogenesis Protocols
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    Chapter 11 Spheroid-Based In Vitro Angiogenesis Model
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    Chapter 12 Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
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    Chapter 13 Angiogenesis Protocols
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    Chapter 14 A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
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    Chapter 15 Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis
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    Chapter 16 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
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    Chapter 17 Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
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    Chapter 18 Angiogenesis Protocols
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    Chapter 19 Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
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    Chapter 20 Angiogenesis Protocols
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    Chapter 21 Angiogenesis Protocols
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    Chapter 22 Models of Oxygen Induced Retinopathy in Rodents
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    Chapter 23 The Sponge Implant Model of Angiogenesis
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    Chapter 24 Angiogenesis Protocols
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    Chapter 25 Angiogenesis Protocols
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    Chapter 26 Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature
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    Chapter 27 Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos
Attention for Chapter 2: Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
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Chapter title
Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
Chapter number 2
Book title
Angiogenesis Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3628-1_2
Pubmed ID
Book ISBNs
978-1-4939-3626-7, 978-1-4939-3628-1
Authors

Daniel J. Royston, Steven Clasper, David G. Jackson

Abstract

The field of lymphatic research has benefited enormously from the discovery of "marker" proteins that permit not only the identification and quantitation of lymphatic vessels in tissue sections for tumor pathology but also the isolation of primary lymphatic endothelial cells for basic research. This chapter focuses on the use of these markers for the immunohistochemical analysis of lymphangiogenesis in both frozen and paraffin-embedded tissue sections and discusses current protocols including newer versions employing biotin tyramide amplification and their associated problems.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 40%
Researcher 1 20%
Student > Postgraduate 1 20%
Unknown 1 20%
Readers by discipline Count As %
Medicine and Dentistry 2 40%
Immunology and Microbiology 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Unknown 1 20%