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Angiogenesis Protocols

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Cover of 'Angiogenesis Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Angiogenesis Protocols
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    Chapter 2 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
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    Chapter 3 Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis
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    Chapter 4 Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
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    Chapter 5 Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
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    Chapter 6 Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells
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    Chapter 7 Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
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    Chapter 8 In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis
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    Chapter 9 Tube-Forming Assays
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    Chapter 10 Angiogenesis Protocols
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    Chapter 11 Spheroid-Based In Vitro Angiogenesis Model
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    Chapter 12 Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
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    Chapter 13 Angiogenesis Protocols
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    Chapter 14 A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
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    Chapter 15 Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis
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    Chapter 16 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
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    Chapter 17 Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
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    Chapter 18 Angiogenesis Protocols
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    Chapter 19 Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
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    Chapter 20 Angiogenesis Protocols
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    Chapter 21 Angiogenesis Protocols
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    Chapter 22 Models of Oxygen Induced Retinopathy in Rodents
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    Chapter 23 The Sponge Implant Model of Angiogenesis
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    Chapter 24 Angiogenesis Protocols
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    Chapter 25 Angiogenesis Protocols
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    Chapter 26 Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature
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    Chapter 27 Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos
Attention for Chapter 12: Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
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Chapter title
Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
Chapter number 12
Book title
Angiogenesis Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3628-1_12
Pubmed ID
Book ISBNs
978-1-4939-3626-7, 978-1-4939-3628-1
Authors

Fatemeh Sharifpanah, Heinrich Sauer

Abstract

Angiogenesis is a complex process of critical importance during development and in physiological and pathophysiological conditions. There is considerable research interest in studying the angiogenesis cascade and consequently a need for a physiologically valid, quantitative, and cost-effective assay. In this chapter, we describe the stem cell spheroid-based sprout assay in three-dimensional fibrin scaffold which allows fast and easy screening of pro- and anti-angiogenic effects of substances with a high degree of reproducibility.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 43%
Professor 1 14%
Student > Postgraduate 1 14%
Student > Doctoral Student 1 14%
Unknown 1 14%
Readers by discipline Count As %
Medicine and Dentistry 4 57%
Agricultural and Biological Sciences 1 14%
Biochemistry, Genetics and Molecular Biology 1 14%
Unknown 1 14%