Chapter title |
Angiogenesis Protocols
|
---|---|
Chapter number | 18 |
Book title |
Angiogenesis Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3628-1_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3626-7, 978-1-4939-3628-1
|
Authors |
Tozer, Gillian M, Prise, Vivien E, Cunningham, Vincent J, Tozer, Gillian M., Prise, Vivien E., Cunningham, Vincent J., Gillian M. Tozer, Vivien E. Prise, Vincent J. Cunningham |
Abstract |
The rate of blood flow through a tissue (F) is a critical parameter for assessing the functional efficiency of a blood vessel network following angiogenesis. This chapter aims to provide the principles behind the estimation of F, how F relates to other commonly used measures of tissue perfusion, and a practical approach for estimating F in laboratory animals, using small readily diffusible and metabolically inert radio-tracers. The methods described require relatively nonspecialized equipment. However, the analytical descriptions apply equally to complementary techniques involving more sophisticated noninvasive imaging.Two techniques are described for the quantitative estimation of F based on measuring the rate of tissue uptake following intravenous administration of radioactive iodo-antipyrine (or other suitable tracer). The Tissue Equilibration Technique is the classical approach and the Indicator Fractionation Technique, which is simpler to perform, is a practical alternative in many cases. The experimental procedures and analytical methods for both techniques are given, as well as guidelines for choosing the most appropriate method. |
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